Buffalo City is a city in Buffalo County, Wisconsin, United States. The population was 1,023 at the 2010 census. The name may be derived …Buffalo City is a city in Buffalo County, Wisconsin, United States. The population was 1,023 at the 2010 census. The name may be derived from a buffalo animal husbandry operation in the past. The city was chartered in 1859. At that time it was the only city in Buffalo County.