Western blots do not determine the absolute abundance of a target protein in a sample but rather the presence or absence of the target protein in a sample, as well as the relative abundance of the ...

Qdot Western Blotting Kits are designed to be a 'plug-and-play' substitute for colorimetric or chemiluminescent detection systems on blots labeled with rabbit and mouse primary antibodies.

For example, if you’re using ... it may be a good idea to run a western blot first to make sure the antibody detects the correct protein and is not creating off-target signals.