Western blot sample preparation: Protein extraction and quantification. Sample preparation is a crucial first step in performing an accurate western blot. 4 After extracting total protein from the ...

Researchers from Germany’s Natural and Medical Sciences Institute describe DigiWest, a multiplexed Western blot protocol that uses Luminex’ bead-based technology and expedites protein profiling.

Figure 4 depicts a three-color Western blot, in which the detected antibodies were labeled with CyDyes™ (GE Healthcare) and fluorescein (Vector Laboratories).