Quantitative polymerase chain reaction ... qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes ...

There are many popular chemistries for real-time PCR. One class uses different fluorescent dyes incorporated in short oligonucleotide probes specific for the amplified target. The second class ...

Dubbed quantitative real-time PCR (qPCR), this technique makes it possible to measure the amount of DNA produced during each PCR cycle. This refinement involves the use of fluorescent dyes or ...

The department’s molecular core facility is fully staffed and equipped with shared instrumentation that includes a quantitative real-time PCR (qRT-PCR ... an inverted upright fluorescent microscope ...