A variety of methods that utilize isothermal amplification (DNA amplification at a constant temperature) can solve this dilemma. The two most popular methods, whole genome amplification (WGA) and loop ...

Despite its utility, PCR’s main limitation is that it requires precise ... The researchers didn’t originally set out to develop a new isothermal amplification method, said Piepenburg. Instead, they ...

For the first kind of test, there are two options: PCR and isothermal amplification technologies. PCR stands for polymerase chain reaction, which is a method for rapidly making copies of a strand of ...

Isothermal amplification techniques, such as Loop-Mediated Isothermal ... Unlike traditional polymerase chain reaction (PCR), ...

However, PCR is limited in its accessibility for rapid on-site testing. Isothermal amplification, unlike PCR, doesn’t require expensive thermal cyclers, making it more accessible. Combining isothermal ...

Traditional PCR amplification is a qualitative assay ... One emerging technique, called isothermal amplification, can amplify nucleic acids at a constant temperature, eliminating the need for ...

Rolling-circle amplification (RCA) driven by DNA polymerase can replicate circularized oligonucleotide probes with either linear or geometric kinetics under isothermal conditions. In the presence ...

The authors developed a one-pot isothermal detection method based on rolling circle amplification (RCA ... and verified to be as accurate as RT-PCR. The principle of ROA is as follows.

for loop-mediated isothermal amplification. LAMP probably deserves an article of its own to explain the process, but suffice it to say that like PCR, LAMP amplifies nucleic acid sequences ...

The existing RT-PCR test systems are time consuming and labor-intensive, with a very high risk of contamination primarily due to post-PCR handling leading to carry over. In addition, the majority ...