Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, and reaction buffer. Scientists must ensure that all reagents in the reaction are ...
1| Combine the following components of the master mix on ice and mix thoroughly: 10× KLA reaction buffer (pH 9.2): 500 mM Tris base, 160 mM (NH 4) 2 SO 4, 25 mM MgCl 2, 1% Tween 20. Note ...
These beads contain buffer salts, nucleotides and Taq polymerase in a lyophilized powder. All that must be added is water, primer and template to a total volume of 25 µl. Check that the bead in each ...
engineered proofreading DNA polymerase, providing superior fidelity and specificity to your PCR. With >300x Taq fidelity and buffer specially formulated for primer annealing at 60°C, Platinum SuperFi ...