This powerful approach to cutting and pasting DNA molecules is known as DNA cloning or recombinant DNA technology. When they act on a DNA molecule, restriction enzymes produce "blunt" ends when ...

blunt and perhaps containing sites for restriction enzymes. 'Old-fashioned' cloning still works, and for years, researchers relied on using cloning methods in which vector and insert were ...

The following is a summary of “Cobalt chloride-mimicked hepatocyte cell hypoxia induces TREX1 leading to Hepatitis B virus restriction,” published in the January 2025 issue of Infectious Disease by ...

This process is referred to as cloning. It uses restriction enzymes to cut the plasmid and the gene fragment at sequence-specific locations that depend on the enzyme used. Then, enzymes called ligases ...