Viral reverse transcriptase (RT ... In contrast to retroviruses, bacterial viruses (bacteriophages) use RT not for genome replication, but for viral gene variation within diversity-generating ...

The assay is routinely used in testing for viral, bacterial ... six primers, which form loop structures to facilitate subsequent rounds of amplification. It can accommodate a reverse transcription ...

The two techniques use the same process except that RT–PCR has an added step of reverse transcription of RNA to DNA, or RT, to allow for amplification. This means PCR is used for pathogens, such as ...

In order to fully understand what this means, it is first necessary to examine the mechanisms of RNA transcription in more detail. In bacteria, all transcription is performed by a single type of ...