One of these new methods, known as structured illumination microscopy (SIM), is highly prized by researchers because it produces high-resolution and high-contrast images with low photon exposure.

This is especially true when studying intricate details beyond the optical resolution limit using structured illumination ...

We acknowledge the Light Microscopy Core Facility, Porter B047, B049, B051 and B059 at the University of Colorado Boulder (RRID:SCR_018993) for help and advice with structured illumination microscopy.

Optical sectioning enables precise 3D imaging by extracting signals in focus and eliminating out-of-focus backgrounds.

Structured-illumination microscopy (SIM) is a practical microscopy technique that explicitly applies the frequency-space principles outlined in this article (Fig. B1a–h). The specimen is ...

It offers high temporal resolution but requires specialized laser systems and fluorophores. Structured Illumination Microscopy uses patterned illumination to encode high-frequency spatial information ...

Super-resolution microscopy techniques, such as stimulated emission depletion (STED) microscopy, structured illumination microscopy (SIM), and single-molecule localization microscopy (SMLM), enable ...