qPCR, also known as real-time PCR ... The primer ends should be GC-rich to allow efficient template binding and high melting temperatures. Design primers with 40-60 percent GC content to enable primer ...
The ubiquity of quantitative PCR (qPCR) for interrogating gene expression in a variety of research and clinical contexts makes it an indispensable laboratory tool. Among the most critical variables ...
Provide the manufacturing team with your precise instructions using this Excel template today ... for gene expression and qPCR assays, and LGC sends you a unique, dual-labeled LNA probe and ...
3). Figure 3: ABsolute Fast QPCR Master Mixes deliver accurate and highly reproducible QRT-PCR data across a large template dynamic range. Ginzinger, D. Gene quantification using real-time ...
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