Learn about quantitative PCR (qPCR), including its fundamentals, common applications, modes of detection, and key quantification methods.

For RT-qPCR, RNA quality and purity is crucial for success and can affect first-strand cDNA synthesis efficiency. You can use total RNA or poly (A)+ RNA as the template, but both must be intact ...

Some protocols include a 'preamplification' step to increase the amount of template for a subsequent qPCR experiment. This even allows qPCR to be conducted on single cells, but it also increases ...

In this article, we will consider how qPCR works, experimental requirements, data analysis and applications of the technique.

How can you identify which technique is best suited to your research requirements? This article explores the advantages of qPCR and dPCR, considering specific applications and deliverable metrics to ...

The ubiquity of quantitative PCR (qPCR) for interrogating gene expression in a variety of research and clinical contexts makes it an indispensable laboratory tool. Among the most critical variables ...

RT-qPCR employs four principal steps: Sample extraction from cells or tissue in a rigorous and reproducible manner; RNA isolation and purification; Reverse transcription to convert the mRNA to ...

Locked nucleic acid (LNA) probes are modified RNA bases where the ribose is locked, thus fixing a specific conformation. They enable shorter probes for qPCR, thereby improving assay sensitivity ...

Pune, March 24, 2023 (GLOBE NEWSWIRE) -- SNS Insider reports states that the Real-Time PCR (qPCR) Market had a valuation of USD 5.2 billion in 2022 and is projected to achieve USD 8.07 billion by ...

UltraMarathonRT® RT-qPCR Kit offers unmatched data reliability and sensitivity for gene expression studies, streamlining workflows and preserving RNA integrity.