The tumor suppressor p53 is a transcription factor that controls the expression of hundreds of genes. Emerging evidence suggests that the p53-induced RNA-binding protein ZMAT3 is a key splicing ...

Readthrough efficiency is often measured with dual reporter systems where the test stop codon and its surrounding nucleotides are flanked by an upstream reporter (often Renilla luciferase; R-luc) and ...

Ten of 11 GBM tumor samples identified as MGMTp methylated by the clinical pyrosequencing assay were also identified as methylated by the ddPCR assay. Five of 23 clinically unmethylated tumors were ...

The dTAG protein degradation assay utilizes a dual luciferase reporter system to measure target protein degradation. One luciferase is used as a house-keeping marker that is unaffected by dTag ...

Figure 2. Promega's DLRTM Assay System. As the sun goes down you see the recurring yet sporadic glow of fireflies around you. The flash of the common firefly (Photinus pyralis) is behavioral display ...

Angle plc, a world-leading liquid biopsy company with innovative circulating tumour cell (CTC) solutions for use in research, drug development and clinical oncology, has announced new, successful ...

Background and aims: CREB (cAMP response element binding protein) transcription factors are key regulators of homeostatic functions in the liver, and CRE binding is increased in hepatic inflammation.

To tackle this possible problem, Wang’s team has developed lysine-free luciferase tags (ACS Med. Chem. Lett., 2024, DOI: 10.1021/acsmedchemlett.4c00271), which he says eliminate the risk of ...