In a study published in Nature, researchers report the successful in vitro reconstitution of meiotic DNA double-strand breaks ...

We contend that microfluidic droplets offer a tractable platform for developing and standardizing a single-cell enzyme activity assay specifically adapted to handle the high diversity and ...

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, Changchun 130022, China ...

It is a unique member of the plastic family as it can be broken down into its constituent units (or monomers) by PETases, which are enzymes that degrade PET. Until now, scientists have discovered ...

and functional assays to rapidly map fitness landscapes across protein sequence space and optimize enzymes for multiple, distinct chemical reactions.” “We’ve developed a computational ...

Rubisco is the enzyme responsible for carbon fixation in plants, algae and many bacteria. A screen of a comprehensive library of rubisco variants (each with a different single-amino-acid mutation ...

Drug-resistance amino acids known in HIV-1 [36] are shown in bold. Amino acids that are the same as those of the reference HIV-1 HXB2 are shown as bars. † Consensus amino acid sequences were ...

Temperature affects enzyme-catalysed reactions by influencing the rate at which they occur. For a reaction to occur between an enzyme and substrate the two molecules need to collide. At lower ...

gracilirostris and has been optimized to produce a luciferase enzyme subunit with improved luminescence and stability (Hall et al., 2012). BRET assays have traditionally employed GFP variants (e.g., ...