Histone deacetylase 6 (HDAC6) is linked with various cellular functions, such as gene expression and protein degradation, as well as many diseases, including breast cancers and Alzheimer’s disease.

Trimethylation of histone H3 at lysine 4 (H3K4me3), is a type of histone modification that usually occurs during active gene transcription when the cell reads the gene to make a protein.

In particular, bottom-up proteomics addresses the following quantification issues: (i) high amino acid sequence similarity between certain histone variants (e.g., 96% identity between human H3.1 and ...