This study presents a valuable finding on the alterations in the autophagic-lysosomal pathway in a Huntington's disease model. The evidence supporting the claims of the authors is solid. However, the ...
LC3-II is used as a marker of autophagy because its lipidation and specific recruitment to autophagosomes provides a shift from diffuse to punctate staining of the protein and increases its ...
The roles of key proteins such as the microtubule-associated protein 1 light chain 3 (LC3), beclin 1, and p62 in autophagy initiation and substrate recognition were also explored. Further ...
The bottom LC3 blots represent a repeated immunoblotting experiment ... grouped according to the functions of the proteins in the autophagy process: autophagy induction signaling (A), membrane ...
Double-Labeled Adenovirus mRFP-GFP-LC3 Transfection and Autophagy Detection The L-02 hepatocytes were seeded and cultured in 12-well plates for 48 h, and then mRFP-GFP-LC3 adenovirus (GV540, Genechem, ...
Representative western blot of PI3K III related autophagy protein SQSTM1/p62 and LC3, and the corresponding quantitative analyses. β-actin was used as loading control. Data were presented as means ± ...
Recycling takes place in our cells at all times: in a process called autophagy, cell components that are no longer needed are enclosed by membranes and broken down into their basic building blocks.
While autophagy is relevant to many biological processes and often serves as a gatekeeper of homeostasis, when something goes wrong it can either lead to disease or be activated to fight against it.
1Cellular Degradation Biology Center, College of Medicine, Seoul National University, Seoul, Republic of Korea. 2Department of Biomedical Sciences, College of Medicine, Seoul National University, ...
LC3 (Little Computer 3) assembly programs that I created for "Introduction to Computing" with Dr. Yale N. Patt Not all of these programs are perfect solutions! LAB-4: NIM is the main project in this ...
7 International Research Fellow of Japan Society for the Promotion of Science (Postdoctoral Fellowships for Research in Japan (Standard)), Tokyo, Japan Correspondence to Dr Shinji Saitoh, Department ...