After cDNA quantification, 200–300 bp target fragments were amplified by PCR using Phusion DNA polymerase (New England Biolabs, Ipswich, MA, USA) for 15 PCR cycles. The libraries were paired-end ...

Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Sequencing adapters were then ligated to the ends of the cDNA fragments. PCR was performed with Phusion High ...

Once scientists have collected an eDNA sample, they analyze it via bar coding, which can either look for a single species or ...

These useful findings assigned a novel functional implication of histone acylation, crotonylation. Although the mechanistic insights have been provided in great detail regarding the role of the YEATS2 ...

This valuable work presents how PRDM16 plays a critical role during colloid plexus development, through regulating BMP signaling. Solid evidence supports the context-dependent gene regulatory ...

Learning about your genetics and ancestry is easier than ever. All you need to do is choose from the many DNA ancestry tests available online, receive your kit and send back the required DNA samples.

1 Division of DNA Repair and Genome Stability, Department of Radiation Oncology, Dana-Farber/Brigham and Women's Cancer Center, Harvard Medical School, Boston, MA, USA 2 2Division of Medical ...

To better understand their pathogenesis, it is necessary to identify the functional roles of these variants, which are largely located in non-coding DNA regions. Here, we employ a human mesencephalic ...

Objective The microbiome contributes to the pathogenesis of inflammatory bowel disease (IBD) but the relative contribution of different lifestyle and environmental factors to the compositional ...

For troubleshooting, configuration and tips, please also refer to the above documentation. For further support, please refer to the Phusion Passenger support page ...