Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, and reaction buffer. Scientists must ensure that all reagents in the reaction are ...

PCR or Polymerase Chain Reaction is a revolutionary method that is used to amplify specific segments of DNA or RNA. This method was developed by Kary Mullis in the 1980s. Kary Mullis received the ...

With >300x Taq fidelity and buffer specially formulated for primer annealing at 60°C, Platinum SuperFi II DNA Polymerase offers efficiency and simplicity in PCR applications requiring highest PCR ...

To make the PCR reaction more simple to prepare, we will be using Ready-to-Go PCR beads from GE Healthcare. These beads contain buffer salts, nucleotides and Taq polymerase in a lyophilized powder.

Phusion High-Fidelity DNA polymerase with 5× Phusion HF buffer (Finnzymes, cat. no. F-530) Critical A high-fidelity DNA polymerase is required for CPEC cloning and the Phusion enzyme works very ...

It is important to also optimize other reaction components, including MgCl 2, dNTPs, and the buffer. Magnesium interacts with dNTPs, primers, the template, and the polymerase to facilitate dNTP ...

The KOD DNA polymerase's optimum temperature (75 o C) and mutation frequency (3.5 x 10-3) is similar to those of Pfu DNA polymerase, but offers an ~5 times higher elongation rate (100-130 ...

“Our platform… uses a co-transcriptional capping strategy that utilizes T7 RNA polymerase and Vaccinia capping enzymes to synthesize capped mRNA in one pot,” Alison Obinna Nwokeoji, PhD, a ...