Several components are involved in a PCR including template DNA, forward and reverse primers, DNA polymerase enzyme, and reaction buffer. Scientists must ensure that all reagents in the reaction are ...

1| Combine the following components of the master mix on ice and mix thoroughly: 10× KLA reaction buffer (pH 9.2): 500 mM Tris base, 160 mM (NH 4) 2 SO 4, 25 mM MgCl 2, 1% Tween 20. Note ...

RNA polymerase is an enzyme that is responsible for copying a DNA sequence into an RNA sequence, duyring the process of transcription. As complex molecule composed of protein subunits, RNA ...