Then, RNA was reversely transcribed into cDNA according to a reverse transcription kit (Transgen Biotech, China). Then 20 µL PCR mixture containing 1 µL cDNA, 8 µL RNase-free water, 0.5 µL forward, ...

It is an ester-derived prodrug that is converted in vivo by serum and tissue esterases to tenofovir, an acyclic nucleoside phosphonate (nucleotide) that inhibits HIV reverse transcriptase.

To examine this hypothesis, bacterial ... The PCR primers consisted of three components: 5′-[454 GS FLX adapter A/B]+[4 nt barcode (aka MID)]+[forward/reverse gene-specific PCR primer]-3′.

Abstract Research of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) and Fibromyalgia (FM), two acquired chronic illnesses affecting mainly females, has failed to ascertain their frequent ...

In bacteria, researchers have long known that transcription begins when RNAP clamps onto a DNA strand and initiates the process after receiving the green light from sigma proteins. But the details ...

reverse transcription at 55°C for 15 min; (2) a pre-denaturation step at 95°C for 30 s; (3) 10 s at 95°C for and 30 s at 60°C for 45 cycles. 2.7. The analysis of primers and sgRNA sequences The ...

All living things rely on RNAP to transcribe DNA into RNA. In bacteria, researchers have long known that transcription begins when RNAP clamps onto a DNA strand and initiates the process after ...

A new study from Oregon Health & Science University has uncovered how small molecules within bacteria interact with ... key proteins such as enzymes and transcription factors.