Like PCR, qPCR amplifies DNA using a DNA polymerase enzyme.This adds new nucleotides to the 3’-OH end of a short, single-stranded DNA segment, called a primer, which complements the template strand. 2 ...

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...

Click image to enlarge. 4. When reporting qPCR results, review the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, as described by Bustin, et al. 3 ...

Some protocols include a 'preamplification' step to increase the amount of template for a subsequent qPCR experiment. This even allows qPCR to be conducted on single cells, but it also increases ...

They enable shorter probes for qPCR, thereby improving assay sensitivity and targeting. Locked ... Provide the manufacturing team with your precise instructions using this Excel template today.

The ubiquity of quantitative PCR (qPCR) for interrogating gene expression in a variety of research and clinical contexts makes it an indispensable laboratory tool. Among the most critical variables ...

The UltraMarathonRT ® Two-Step RT-qPCR Kit is also the ideal companion for validating novel genes detected in RNA-seq datasets, offering compatibility with RNA templates of all types, including ...

The MIQE guidelines and papers such as the one discussed here can be applied to avoid pitfalls. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) is everywhere. Its ubiquity ...

Identify the principles and advantages of microfluidic singleplex qPCR technology, including its scalability, throughput, and ability to simultaneously detect multiple pathogens. Learn how ...