A375 and A2058 cells were transfected for 24 h with an adenovirus containing the mCherry-eGFP-LC3B gene (Suzhou Genepharma, China), a marker used for tracking autophagy flux. After transfection, the ...

Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou 225001, China Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and ...

Get Instant Summarized Text (Gist) Autophagy, particularly through the protein ATG5, plays a crucial role in maintaining brain health by regulating glucose metabolism in neurons. In Purkinje cells ...

1Cellular Degradation Biology Center, College of Medicine, Seoul National University, Seoul, Republic of Korea. 2Department of Biomedical Sciences, College of Medicine, Seoul National University, ...

These results suggest that, in the recruitment of K63 and K27 ubiquitin chains to PDE9A, CHIP triggers its sequential ubiquitination and subsequent degradation via the autophagy–lysosomal pathway.

Henan Key Laboratory of Environmental and Animal Product Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, Henan,China ...

Autophagy and inflammation play determinant roles in the pathogenesis ... permeabilized in PBS containing 0.1% Triton X-100 and then incubated for 1.5 h at 37°C with anti-LC3B (1:200), anti-SQSTM1/p62 ...