University of Virginia scientists discover new cell organelle called "hemifusome" that helps manage cellular recycling.

The NIH-3T3 fibroblast cells were cultured on the scaffolds to study the effects of the released EGF by using the Alamar Blue Cell Viability Assays, fluorescence image analysis, and SEM. The animal ...

The stability of EGF was evaluated using reversed phase high-performance liquid chromatography (RP-HPLC). Cells were cultivated for 3 days to further estimate the impact of antioxidants on cell ...

By using the microfluidic spinning technology we generated tiny hydrogel tubular scaffolds. Fibroblast (NIH/3T3) cell cultures were performed for seventeen days to demonstrate the potential of cell ...

A flow cytometry assay was developed to detect changes in phosphoRab10 levels in pools of mouse NIH-3T3 cells harboring unique CRISPR guide sequences. Multiple negative and positive regulators were ...

A software, based on the processing of these images, computes DEP spectra and calculates the electric parameters of the cells. The platform has been used for acquiring and processing DEP spectra of ...

Lithographic nanopatterning techniques such as photolithography, electron-beam lithography, and nanoimprint lithography (NIL) have revolutionized modern-day electronics and optics. Yet, their ...

Leveraging the CellRaft AIR ® System emerged as the preferred method for identifying clonal NIH-3T3 cells that were double positive for both transgenes. Figure 1. Cell Line Development Workflow.

Adhesion and proliferation behaviors of fibroblast cells (NIH-3T3) on sterile gelatin microspheres were investigated. Microspheres were placed in a non-treated 48-well plate (50 mg/well), whereas a ...

NIH/3T3 cell lines stably expressing human native or mutant LAT1 were established. They confirmed that endogenous mouse CD98hc forms a disulfide bond with exogenous human LAT1 in naLAT1/3T3, but ...

Western blot analysis of CRTH2 expression in cell membrane and ER in NIH 3T3 and HEK293T cells. Myc‐tagged CRTH2‐expressing plasmid was transfected into NIH 3T3 and HEK293T cells. Whole‐cell lysate, ...

NIH-3T3 cells (4 × 10 4 cells/well) were plated in a 96-well plate for 24 h then culture medium was discarded and replaced with DMEM containing 0.4% NBS followed by incubation of cells for another 24 ...