After cDNA quantification, 200–300 bp target fragments were amplified by PCR using Phusion DNA polymerase (New England Biolabs, Ipswich, MA, USA) for 15 PCR cycles. The libraries were paired-end ...

Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Sequencing adapters were then ligated to the ends of the cDNA fragments. PCR was performed with Phusion High ...

Once scientists have collected an eDNA sample, they analyze it via bar coding, which can either look for a single species or ...

A new study in the journal Nature throws a new theory into the fray. Analyzing a wealth of DNA collected from fossilized human bones, the researchers found that the first Indo-European speakers ...

This valuable work presents how PRDM16 plays a critical role during colloid plexus development, through regulating BMP signaling. Solid evidence supports the context-dependent gene regulatory ...

Learning about your genetics and ancestry is easier than ever. All you need to do is choose from the many DNA ancestry tests available online, receive your kit and send back the required DNA samples.

1 Division of DNA Repair and Genome Stability, Department of Radiation Oncology, Dana-Farber/Brigham and Women's Cancer Center, Harvard Medical School, Boston, MA, USA 2 2Division of Medical ...

To investigate this in more detail, we measured EB progeny production using an inclusion forming unit (IFU) assay and genomic DNA levels (a proxy for total bacteria, i.e., EB+RB) by qPCR. To quantify ...

To better understand their pathogenesis, it is necessary to identify the functional roles of these variants, which are largely located in non-coding DNA regions. Here, we employ a human mesencephalic ...

Ancient DNA locates the geographic origin of Proto-Indo-Europeans and Proto-Anatolians and describes their early migrations out of the Caucasus Lower Volga and North Pontic regions of present-day ...