Pulsed field gradients (PFGs), also known as gradient enhanced spectroscopy (GES), can be used to achieve coherence selection and minimize the need for extensive phase cycling.

A good starting point for work is a scouting gradient. The conditions recommended by John Dolan are 5–95% acetonitrile, low pH, and are dependent on the column length.

Changes in liquid composition during gradient elution liquid chromatography (LC) coupled to mass spectrometry (MS) analyses affect the electrospray operation. To establish methodologies for …

This overview offers a look at gradients and how they are developed. We will also discuss common pitfalls and how to address them. Why Use Gradients? Gradients are useful for: - …

Most proteomics laboratories use a simple linear analytical gradient for nano-LC-MS/MS experiments, though recent evidence indicates that optimized non-linear gradients result in …

Generally, organic solvents for HPLC, such as acetonitrile and methanol, are available in three qualities: Isocratic grade, gradient grade and hypergrade for LC-MS LiChrosolv ®. For MS …

In this study we assess the two chromatographic parameters: column length and gradient length in ranges that are typically used for high throughput shotgun proteomics. Our results suggest …

Apr 1, 2016 · Capillary HPLC (cLC) with gradient elution is the separation method of choice for the fields of proteomics and metabolomics. This is due to the complementary nature of cLC …

Apr 10, 2014 · Most proteomics laboratories use a simple linear analytical gradient for nano-LC-MS/MS experiments, though recent evidence indicates that optimized non-linear gradients …

Nov 21, 2014 · We describe a new approach called “Measure Your Gradient” that potentially solves these problems. One runs a test mixture containing 20 standards on a standard …