Feb 10, 2024 · Fluorescent Neuronal Cells v2 is a collection of fluorescence microscopy images and the corresponding ground-truth annotations, designed to foster innovative research in the domains of Life...

Oct 9, 2019 · Using a fluorescent probe that lights up when brain cells are electrically active, MIT and Boston University researchers have shown that they can image the activity of many neurons at once, in the brains of mice.

Mar 12, 2021 · In this article, we review the most recent methodological and technological advances that now allow us to label and report the localization and function of various targets at different scales, from...

The asymmetric cyanine derivative with a PEG linker enhances cellular uptake, protects the fluorescent dye from degradation, and improves the mitochondria-targeting capacity, making it suitable for imaging applications focusing on visualizing mitochondria in brain cells.

Jan 6, 2022 · We discuss advances in fluorescent indicators of neural activity, viral and genetic methods to express these indicators, chronic animal preparations for long-term imaging studies, and microscopes to monitor and manipulate the activity of large neural ensembles.

3 days ago · Substantial levels of fluorescent signals were observed in the brain at the time points ... the tdTomato expression level of these brain cells after three injections was quantified as 16.7 ± 2.2% ...

Sep 18, 2023 · Here we introduce CAptVE, a method using slow off-rate modified aptamers for cell fluorescence labeling on ultrastructurally reconstructable electron micrographs. CAptVE provides labeling for a wide range of biomarkers, offering a pathway to integrate molecular analysis into recent approaches to delineate neural circuits via connectomics.

2 days ago · ExLSFM represents an emerging modality that has only recently been considered for connectomics, yet it has a speed advantage over EM and opens doors to using multiplexed fluorescent markers for simultaneously probing the brain’s molecular information. 14 Each of these technologies deserves consideration in the context of mammalian ...

Aug 22, 2024 · In this review, we outline the current range of fluorescent probes that are available to researchers for ex vivo brain section imaging, including their physical and chemical characteristics, staining targets, and examples of discoveries for which they have been used.

Sep 22, 2022 · Today the National Institutes of Health announces two transformative projects supported by the Brain Research Through Advancing Innovative Neurotechnologies® (BRAIN) Initiative : The BRAIN Initiative® Cell Atlas Network (BICAN) and the Armamentarium for Precision Brain Cell Access .These BRAIN 2.0 projects aim to transform our understanding of brain cell types and the precise tools needed to ...

In this review, we discuss the recent advances in the development of fluorescent transgenic mouse models that overcome to some extent the technical and conceptual limitations and tradeoffs between different strategies. We also discuss the potential use of these strains for understanding disease.

Jan 6, 2022 · We discuss advances in fluorescent indicators of neural activity, viral and genetic methods to express these indicators, chronic animal preparations for long-term imaging studies, and microscopes to monitor and manipulate the activity of large neural ensembles.

Jan 17, 2023 · Rice’s Han Xiao, Stanford’s Zhen Cheng and collaborators have developed a new tool for noninvasive brain imaging that can help illuminate hard-to-access structures and processes. Their small-molecule dye, or fluorophore, is the first of its kind that can cross the blood-brain barrier.

Oct 21, 2020 · Here, we describe a rapid synthetic route to generate a series of nuclear stains that enable experiments with live brain cells (i.e., water solubility, cell permeability, high quantum yield, high photostability, and most important, negligible cytotoxicity).

These sensors have been targeted to specific brain regions and cell types in different organisms to track multiple metabolic processes at single cell (and subcellular) resolution. Here, we review genetically encoded biosensors used to study metabolism in the brain.

Oct 20, 2019 · Using a fluorescent probe that lights up when brain cells are electrically active, MIT and Boston University researchers have shown that they can image the activity of many neurons at once, in the brains of mice.

Feb 11, 2025 · Then, the tissue is washed with fluorescent DNA probes that are complementary to the target mRNA sequences. These probes can then be imaged and sequenced, revealing the locations of each mRNA sequence within a cell. This allows for the visualization of mRNA molecules that encode thousands of different genes within single cells.

Jan 17, 2023 · Rice's Han Xiao, Stanford's Zhen Cheng and collaborators have developed a new tool for noninvasive brain imaging that can help illuminate hard-to-access structures and processes. Their...

Feb 6, 2025 · By using brain tissue from patients with cancer, who had been administered bromodeoxyuridine (BrdU) — a DNA-labeling compound — we identified dividing progenitor cells in the dentate gyrus ...

Jan 9, 2025 · Two human cells making RNA made visible by the bioluminescent lantern in pink (middle image). On the left, the same cells making a fluorescent protein that’s distributed evenly throughout the two cells. The right image is an overlay of the two, emphasizing that the RNA is distributed unevenly and is concentrated at specific locations.