In this guide I will explain how to create a linear standard curve using Microsoft Excel and how to use it to calculate unknown sample values. I will use the BCA total protein assay standards as an example.

To create a standard curve, it is recommended to start with the undiluted cDNA sample as your first point. From this, you need to create a serial dilution series. A 1:10 dilution is commonly used to create a standard curve with at least 5-points.

In this tutorial, I will show you how to interpolate a standard curve by using GraphPad Prism to calculate concentrations from an ELISA experiment.

Below is a PCR primer efficiency calculator. All that is needed for the calculator is the slope of the standard curve during the serial dilutions during the qPCR. Once entered, hit the calculate button and the primer efficiency (as a percentage) and the amplification factor (E) will be returned. Mastering qPCR

The FREE Pfaffl Method Excel template. For those still struggling with the analysis, or just want an easy template to use to quickly calculate the gene expression ratio using the Pfaffl method, I have created a Microsoft Excel template to freely download.

If you would like to calculate the concentration in pg/mL, you can use these values when preparing your standard curve. The end result, after interpolating the standard curve will then be in pg/mL. I hope that makes sense.

In this guide, I will show you how to perform the delta-delta Ct method for calculating relative gene expression values in qPCR by using Microsoft Excel.

In this guide, I will show you the incredibly easy way to create Z-scores (standard scores) by using SPSS. It only takes three steps!

RIPA lysis buffer is commonly used to lyse cells and tissues for protein extractions. Learn how to make RIPA lysis buffer by using this simple recipe.

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