Malate synthase is found as an octamer of identical subunits (each roughly 60kDa) in some plants, including maize. It is found as a homotetramer in the fungus Candida and as a homodimer in eubacteria. Malate synthase is fused to the C-terminus of isocitrate lyase in C. elegans, resulting in a single bifunctional

苹果酸合酶（英語： malate synthase ，EC 2.3.3.9）是能催化如下反应的酶: 乙酰-CoA + H 2 O + 乙醛酸 (S)-苹果酸 + CoA. 该酶属于轉移酶中 酰基转移酶 （ 英语 ： acyltransferases ） 的一类，并在转移过程中把酰基转化为烷基。苹果酸合酶参与了乙醛酸循环。

苹果酸合酶 （ 英语 ：malate synthase， EC 2.3.3.9）是能 催化 如下 反应 的 酶: 乙酰-CoA + H 2 O + 乙醛酸 (S)-苹果酸 + CoA 。 该酶属于 转移酶 中 酰基转移酶 的一类，并在转移过程中把 酰基 转化为 烷基 。

Sep 25, 2022 · Two enzymes involved in the glyoxylate cycle are isocitrate lyase (ICL) and malate synthase (MS). We determined the contribution of MS in the survival of S. Typhimurium under carbon limiting and...

Malate can be produced by malate synthase (MAS) and metabolized malic enzyme (ME) or malate dehydrogenase (MDH) (Box 1). Amongst these, only MDH catalyzes a reversible reaction that is coupled to the reduction/oxidation of NAD(P) + /NAD(P)H, while interconverting malate and oxaloacetate (OAA).

Malate synthase G catalyzes the condensation of glyoxylate with acetyl-CoA to produce malate [9]. There is a substantial body of evidence based on structurally and functionally related enzymes suggesting that significant changes in the relative orientation of the four domains of the protein could accompany ligand binding [19,20] .

The unique enzymes of this route are isocitrate lyase (ICL) and malate synthase (MS). ICL cleaves isocitrate to glyoxylate and succinate, and MS converts glyoxylate and acetyl-CoA to malate. The end products of the bypass can be used …

Jul 5, 2011 · Malate synthase catalyzes the Claisen-like condensation of acetyl-coenzyme A (AcCoA) and glyoxylate in the glyoxylate shunt of the citric acid cycle. The Mycobacterium tuberculosis malate synthase G gene, glcB, was cloned, and the N-terminal His 6-tagged 80 kDa protein was expressed in soluble form and purified by metal affinity chromatography ...

Aug 8, 2011 · In this work we examine Mtb malate synthase, from the currently untargeted glyoxylate shunt. We report a steady-state ordered bi bi kinetic mechanism. Additionally, we propose a Mg 2+ centered reaction mechanism in which glyoxylate binds first to the enzyme, coordinating Mg 2+ .

Malate Synthase is an enzyme that catalyzes the condensation reaction between glyoxylate and acetyl-CoA to produce l-malate and CoA-SH, with a high irreversible character. Its activity is typically measured spectrophotometrically by titrating CoA-SH with DTNB or by directly measuring the decrease in optical density at 232 nm.